Journal: Cells

Article Title: FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells.

doi: 10.3390/cells11172730

Figure Lengend Snippet: Figure 1. Differentiation of pluripotent stem cells into dermal papilla cells.

Article Snippet: Dermal papilla cells (iDP) differentiated from human pluripotent stem cells (iPSKYOU (ACS-1023TM, ATCC, USA), iPS-DYP0730 (ACS-1023TM, ATCC, USA), iPSC-DP [21], hES-MK05 (Vavilov Institute of General Genetics, Russian Academy of Sciences) and primary human dermal papilla cells (hDP) from 3 donors were used to create 3D dermal cultures.

Techniques:

Journal: Cell Death & Disease

Article Title: Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment

doi: 10.1038/cddis.2013.88

Figure Lengend Snippet: Characterization of HS-181 cell line after treatment with zebularine. ( a ) RT-PCR of cardiac markers after treatment with zebularine. Myh7, Myh6, Actc, cTnI and Serca2 present more expression after treatment. ( b ) Immunostaining of treated cells to detect cardiac-specific proteins. Scale bars; 50 μ m. ( c ) Blots of Proteome Profiler Array and the resulting quantification histograms demonstrating inhibition of pluripotency marker expression and ( d ) increased levels of mesodermic proteins after zebularine treatment (black arrows)

Article Snippet: Protein expression profiles were assayed using the specific human pluripotent Stem Cell array kit ‘Proteome Profiler Array' (R&D Systems Europe, Abingdon, UK; ARY010) following the manufacturer's instructions.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Inhibition, Marker

Journal: Stem cell research

Article Title: Generation of two familial hypercholesterolemia patient-specific induced pluripotent stem cell lines harboring heterozygous mutations in the LDLR gene

doi: 10.1016/j.scr.2024.103463

Figure Lengend Snippet:

Article Snippet: Ectoderm was inducted with the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems #SC027B).

Techniques: Virus, Modification